翻訳と辞書 Words near each other ・ RIC8A ・ Rica ・ Ribosomal protein S19 ・ Ribosomal protein s6 ・ Ribosomal protein SA ・ Ribosomal rescue factor ・ Ribosomal RNA ・ Ribosomal S15 leader ・ Ribosomal s6 kinase ・ Ribosomal translocation ・ Ribosomal-protein-alanine N-acetyltransferase ・ Ribosomally synthesized and post-translationally modified peptides ・ Ribosome ・ Ribosome biogenesis ・ Ribosome biogenesis protein BRX1 homolog ・ Ribosome display ・ Ribosome profiling ・ Ribosome Recycling Factor ・ Ribosome shunting ・ Ribosome-inactivating protein ・ Ribosome-nascent chain complex ・ Ribosomopathy ・ Ribostamycin ・ Riboswitch ・ Ribosyldihydronicotinamide dehydrogenase (quinone) ・ Ribosylnicotinamide kinase ・ Ribosylpyrimidine nucleosidase ・ Ribot ・ Ribot (horse) ・ Ribot's Law Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Ribosome display ： ウィキペディア英語版 Ribosome display Ribosome display is a technique used to perform ''in vitro'' protein evolution to create proteins that can bind to a desired ligand. The process results in translated proteins that are associated with their mRNA progenitor which is used, as a complex, to bind to an immobilized ligand in a selection step. The mRNA-protein hybrids that bind well are then reverse transcribed to cDNA and their sequence amplified via PCR. The end result is a nucleotide sequence that can be used to create tightly binding proteins. == Ribosome display process == Ribosome display begins with a native library of DNA sequences coding for polypeptides. Each sequence is transcribed, and then translated ''in vitro'' into polypeptide. However, the DNA library coding for a particular library of binding proteins is genetically fused to a spacer sequence lacking a stop codon before its end. The lack of a stop codon prevents release factors from binding and triggering the disassembly of the translational complex. So, this spacer sequence stays attached to the peptidyl tRNA and occupies the ribosomal tunnel, and thus allows the protein of interest to protrude out of the ribosome and fold. What results is a complex of mRNA, ribosome, and protein which can bind to surface-bound ligand. This complex is stabilized with the lowering of temperature and the addition of cations such as Mg2+. During the subsequent binding, or panning, stages, the complex is introduced to surface-bound ligand. This can be accomplished several ways, for example using an affinity chromatography column with a resin bed containing ligand, a 96-well plate with immobilized surface-bound ligand, or magnetic beads that have been coated with ligand. The complexes that bind well are immobilized. Subsequent elution of the binders via high salt concentrations, chelating agents, or mobile ligands which complex with the binding motif of the protein allow dissociation of the mRNA. The mRNA can then be reverse transcribed back into cDNA, undergo mutagenesis, and iteratively fed into the process with greater selective pressure to isolate even better binders.'' 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Ribosome display」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.